Multiscale modelling of biomolecular corona formation on metallic surfaces

• Parinaz Mosaddeghi Amini,
• Ian Rouse,
• Julia Subbotina and
• Vladimir Lobaskin

Beilstein J. Nanotechnol. 2024, 15, 215–229, doi:10.3762/bjnano.15.21

• protein corona composition in the deposited milk layer on aluminum surfaces. We consider a simplified model of milk, which is composed of the six most abundant milk proteins found in natural cow milk and lactose, which is the most abundant sugar found in dairy. Through our study, we ranked selected

Identification of physicochemical properties that modulate nanoparticle aggregation in blood

• Ludovica Soddu,
• Duong N. Trinh,
• Eimear Dunne,
• Dermot Kenny,
• Giorgia Bernardini,
• Ida Kokalari,
• Arianna Marucco,
• Marco P. Monopoli and
• Ivana Fenoglio

Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44

• –protein interaction may lead to bridging among particles, thus promoting agglomeration [23]. In the present study, a set of six silica and carbon NPs of known size and morphology was used to evaluate the effect of the size and surface properties on the protein corona composition, platelet activation and

• composition between the two materials has some similarities when incubated at 10% plasma; however, it becomes highly specific to the NP surface properties at higher concentrations as confirmed by the significant difference in the corona composition. In particular, at 10% of plasma, both NPs preferentially

• formed in the region of 36–30 kDa in addition to a less pronounced band of MW lower than 28 kDa. At higher plasma concentration, the corona composition of the SNP changed significantly where the fibrinogen gel bands were displaced by three predominant bands of 90 kDa and a duplet of 50 kDa, later

Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine

• Valentina Francia,
• Daphne Montizaan and
• Anna Salvati

Beilstein J. Nanotechnol. 2020, 11, 338–353, doi:10.3762/bjnano.11.25

• their surface following administration [52][53]. Thus, controlling the corona composition can possibly provide new ways to control the initial interactions of nano-sized materials with cells. The corona composition depends on nanoparticle physicochemical characteristics, such as size, shape, charge

• , hydrophobicity, rigidity and surface characteristics [3][7][8][48][54]. By changing these properties, the corona composition might be tuned to contain components that bind to specific cell surface receptors and initiate internalization [17][49][55][56][57]. Similarly, artificial coronas can be formed to achieve

• interactions of nanoparticles with cells are being more and more recognized [41][58][59]. For example, multiple attempts have been made in trying to predict how the presence of the corona affects targeting of nanomedicines [60][61]. Similarly, it is known that the corona composition changes not only with

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• concentration. On PLGA NPs incubated with either FBS or human serum a clear difference in qualitative corona protein composition was identified by SDS-PAGE and LC–MS/MS in combination with bioinformatic protein classification. In the case of human serum a considerable change in corona composition was observed

• leading to a concentration-dependent desorption of abundant proteins in conjunction with an adsorption of high-affinity proteins with lower abundance. Cell incubation experiments revealed that the respective corona composition showed significant influence on the resulting nanoparticle–cell interaction

• , and it has been extensively investigated and comprehensively reviewed that the corona composition varies in dependence of the physicochemical properties of the NPs [5][6]. However, it is emerging that the characteristics of the biological environment, e.g., protein concentration [7][8][9], protein

Protein corona – from molecular adsorption to physiological complexity

• Lennart Treuel,
• Dominic Docter,
• Michael Maskos and
• Roland H. Stauber

Beilstein J. Nanotechnol. 2015, 6, 857–873, doi:10.3762/bjnano.6.88

• composition and the latest findings that help to shed light on temporal evolution of the full serum corona for the first time. Finally, we discuss the most recent advances regarding the molecular-scale mechanistic role of the protein corona in cellular uptake of NPs. Keywords: agglomeration; corona

• this is even true for such high affinity interactions is a further indication that denaturation of proteins may be a centrally decisive factor for any non-equilibrium effects observed in corona formation [10]. Particle size determines corona composition For a deeper understanding of protein corona

• ]. They could show that even differences in silica nanoparticle (SiNP) size of only 10 nm significantly affected the protein corona composition around three different SiNPs (with diameters of about 20, 30, and 110 nm). One strategy to interpret their results was to classify the SiNP-specific protein

In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics

• Wolfgang G. Kreyling,
• Stefanie Fertsch-Gapp,
• Martin Schäffler,
• Blair D. Johnston,
• Nadine Haberl,
• Christian Pfeiffer,
• Jörg Diendorf,
• Carsten Schleh,
• Stephanie Hirn,
• Manuela Semmler-Behnke,
• Matthias Epple and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180

• , surface area and curvature of the AuNP [7]. Serum protein binding of 5 nm sized AuNP with different surface modifications In our next attempt we hypothesized that the configuration of the protein corona (composition, size, geometric orientation) depends on the surface ligands present on the AuNP

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

• Dominic Docter,
• Christoph Bantz,
• Dana Westmeier,
• Hajo J. Galla,
• Qiangbin Wang,
• James C. Kirkpatrick,
• Peter Nielsen,
• Michael Maskos and
• Roland H. Stauber

Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151

• the properties of the NP dictate the extent and specificity of protein binding profiles, which are complex, and in line with other studies may well consist of more than hundred different proteins [22][27][28][29]. Albeit we did not perform a detailed identification of the protein corona composition by